An expanded adenylate kinase gene family in the protozoan parasite Trypanosoma cruzi.

BOUVIER, LEÓN ALBERTO; MIRANDA, MARIANA; CANEPA, GASPAR; ALVES, M; PEREIRA, CLAUDIO

BIOCHIMICA ET BIOPHYSICA ACTA (ENZIMOLOGY)

Amsterdam [etc.] Elsevier Pub. Co.

Año: 2006 vol. 1760 p. 913 - 921

0006-3002

Adenylate kinases supply energy routes in cellular energetic homeostasis. In this work, we identified and characterized the adenylate kinase activity in extracts from the flagellated parasite Trypanosoma cruzi, the causative agent of Chagas´ disease. Adenylate kinase activity was detected in different subcellular fractions and the cytosolic isoform was biochemically characterized. Cytosolic adenylate kinase specific activity increases continuously during the epimastigote growth and is down-regulated when other soluble phosphotransferase, arginine kinase, is overexpressed. Six different genes of adenylate kinase isoforms were identified and the mRNA expression was confirmed by RT-PCR and Northern Blot. Three open reading frames coding for different enzyme isoforms named TzADK1, TzADK2 and TzADK5 were cloned and functionally expressed in E. coli. This work reports an unusually large number of genes of adenylate kinases and suggests a coordinated regulation of phosphotransferase-mediated ATP regenerating pathways in the unicellular parasite Trypanosoma cruzi.