Bisphenol S (BPS) induces structural and molecular alterations in uterus and ovaries in a rat model of PCOS

BRENDA DEMACOPULO; CANESSA CAROLINA; FIDYKA AILEN; ZAMORA NOELIA; CABRINI RL; ERICA KREIMANN

Buenos Aires, Argentina

Congreso; Reunión Conjunta de sociedades Científicas, SAIC; 2017

SAIC

SAIC 2017: DemacopuloB_Resumen01Área: ToxicologíaDemacopulo B1,2, Canessa C1,3, Fidyka A1,3, Zamora N1,3, Cabrini RL1,4, Kreimann E1,21Departamento de Radiobiología, Comisión Nacional de Energía Atómica (CNEA), 2Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 3 Universidad CAECE, 4 Facultad de Odontología UBABisphenol S (BPS) induces structural and molecular alterations in uterus and ovaries in a rat model of PCOS Bisphenol A or (BPA) is the most common component of polycarbonates and it has toxic effects in the reproductive system. In order to reduce BPA exposure, the use of alternative compounds such as Bisphenol S (BPS) has been proposed. Unfortunately, BPS also displayed estrogenic, antiestrogenic, androgenic and antiandrogenic actions. Therefore, there is a growing concern about the biological effects of BPS in terms of toxicity, genotoxicity, endocrine disruption and neurobiological levels.Objectives: To study the effect of BPS in the uterus and its role in ovarian cysts development in a known rat model of PCOS.Materials and Methods: Immature Wistar rats (21-22 days old) were injected with vehicle, dehydroepiandrosterone or DHEA (60 mg/kg), DHEA+BPS and BPS alone (1 ug/kg) for 20 days. Tissues were stained with hematoxylin-eosin (H&E) and immunohistochemistry. Statistical differences were evaluated by the Student T-Test.Results: BPS induces alterations in uterus such as an increase in tissue elasticity and vacuolization of endometrial cells. The administration of BPS in DHEA treated rats, increased significantly the percentage of vacuolization of the endometrial layer (DHEA/BPS 68.77%±17.87 compared to DHEA: 40.29%±19.06, p