Biodegradation and Histological Response of PHBV Porous Scaffolds

ELIDA B HERMIDA; I RUIZ; A BALDESSARI; E L KREIMANN; R CABRINI; G JUVENAL; GN HERMIDA; M FUNGUEIRO

Mar del Plata

Taller; Segundo taller de Organos Artificiales, Biomateriales e Ingenieria de Tejidos; 2011

This work presents, on one hand, in vitro tests of porous salt leached scaffolds of polyhydroxybutyrate-co-hydroxyvalerate (PHBV), at 37ºC and pH 7.4 in phosphate-buffered saline (PBS) and PBS with lipase. An alkaline treatment enhances the biodegradation and decrease in the molecular weight does not depend on the presence of lipase. On the other hand, the in vivo tissue reactions were tested by subcutaneous implantation in Wistar rats. Toxicity and inflammatory response at 1 week and 1 month post implantation, evaluated from H&E stained sections shows that the SL scaffolds have higher bioabsorption rate and less inflammatory response.in vitro tests of porous salt leached scaffolds of polyhydroxybutyrate-co-hydroxyvalerate (PHBV), at 37ºC and pH 7.4 in phosphate-buffered saline (PBS) and PBS with lipase. An alkaline treatment enhances the biodegradation and decrease in the molecular weight does not depend on the presence of lipase. On the other hand, the in vivo tissue reactions were tested by subcutaneous implantation in Wistar rats. Toxicity and inflammatory response at 1 week and 1 month post implantation, evaluated from H&E stained sections shows that the SL scaffolds have higher bioabsorption rate and less inflammatory response.in vivo tissue reactions were tested by subcutaneous implantation in Wistar rats. Toxicity and inflammatory response at 1 week and 1 month post implantation, evaluated from H&E stained sections shows that the SL scaffolds have higher bioabsorption rate and less inflammatory response.