Development of an electrophoretic method based on nanostructured materials for HbA1c determination

MARIANA HAMER; NARELLA BASSI; DENISE A. GRELLA

ELECTROPHORESIS

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2018

0173-0835

Glycosylated hemoglobin (HbA1c) detection is performed routinely in hospitals as it is the most widespread confirmatory diagnosis of diabetes mellitus. Here we present a novel CE method for measuring HbA1c by introducing silica nanoparticles (NPs) modified with a boronic acid derivative (sugar loadings of 51 ± 2μg/mg) as pseudo-stationary phase. Before the sample injection, SiO2NP-B(OH)2 were introduced via pressure. Electrophoretic separation was explored through variation of the buffer pH and separation voltage, being the best separation, resolution and shorter separation time achieved with a 25mM phosphate buffer pH 6.5. The calibration curve obtained was expressed as Area= 182.05% x HbA1c ? 377.02; R² = 0.9826, using a UV/VIS absorbance detector at 415 nm (diode array). No interferences were observed from carbamylated or acetylated hemoglobin and the method shows a noteworthy stability. A paired t-test was applied to compare the developed CE method with a commercial HbA1c test and no significant variations have been observed at a 90% significance level.Esta publicación fue elegida para aparecer en la contratapa de la revista. https://doi.org/10.1002/elps.201870062