Insulin receptor activation effects on synaptosomal 2-AG hydrolysis in an amyloidosis model induced by AB oligomers

PASCUAL, A.C.; SALAS, S.R.; PASQUARÉ, S.J.

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencia; 2019

Insulin (Ins) plays an important role in synaptic plasticity and is tightly related to Alzheimer´s disease (AD). Aβ oligomers (AβO), which are responsible for synaptic dysfunction in AD, can bind to Ins receptor (IR) and can therefore be internalized into neurons. AβO also disrupt the synaptic membrane and diminish 2-AG availability, the main neuroprotective cannabinoid. Ins can prevent AβO binding to IR, thus attenuating its neurotoxicity. Here, we hypothesized that Ins prevent AβO deleterious effects on 2-AG metabolism. To this end, we isolated cerebral cortex synaptosomes (syn) by differential centrifugation purified in ficoll gradients and preincubated them with 10 μM LY294002 (phosphatidylinositol-3-kinase -PI3K- inhibitor) or 100 μM genistein (tyrosine kinase inhibitor) for 10 min, and subsequently incubated with 0.2 mM vanadate (protein-tyrosine phosphatase inhibitor), 100 nM Ins, or 0.2 mM vanadate plus 100 nM Ins, for 30 min. Syn were then incubated for 10 min with or without 0.1 μM AβO. After this incubation, activation of IR signaling by Western Blot, released LDH activity, and 2-AG hydrolysis activity were evaluated. It was observed that a 30 min incubation with Ins and vanadate activated IR and Akt (p0.05). As to syn membrane damage, neither of the pretreatments could prevent AβO effect on LDH release (p>0.05). On the other hand, Ins and vanadate decreased 2-AG hydrolysis (p0.05). However, in the presence of AβO, Ins and vanadate failed to alter 2-AG hydrolysis (p>0.05) and LY increased this activity (p