Age-related changes in retinoic, docosahexaenoic and arachidonic acid modulation in nuclear lipid metabolism

GAVEGLIO, V.L.; PASCUAL, A.C.; GIUSTO, N.M.; PASQUARÉ, S.J.

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2016 vol. 604 p. 121 - 127

0003-9861

The aim of this work was to study how age-related changes couldmodify several enzymatic activities that regulate lipid mediator levelsin nuclei from rat cerebellum and how these changes are modulated by alltransretinoic acid (RA), docosahexaenoic acid (DHA) and arachidonic acid(AA). The higher phosphatidate phosphohydrolase activity and lowerdiacylglycerol lipase (DAGL) activity observed in aged animals comparedwith adults could augment diacylglycerol (DAG) availability in theformer. Additionally, monoacylglycerol (MAG) availability could be highdue to an increase in lysophosphatidate phosphohydrolase (LPAPase)activity and a decrease in monocylglycerol lipase activity. Interestingly, RA, DHA and AA were observed to modulate these enzymaticactivities and this modulation was found to change in aged rats. In adultnuclei, whereas RA led to high DAG and MAG production through inhibitionof their hydrolytic enzymes, DHA and AA promoted high MAG production byLPAPase and DAGL stimulation. In contrast, in aged nuclei RA caused highMAG generation whereas DHA and AA diminished it through LPAPase activitymodulation. These results demonstrate that aging promotes a differentnuclear lipid metabolism as well as a different type of non-genomicregulation by RA, DHA and AA, which could be involved in nuclearsignaling events.