Neonatal obesity: implications for memory impairment and neurosteroidogenesis dysregulation.

ROSSETTI MF; SCHUMACHER ROCÍO; CANESINI G; FERNÁNDEZ PR; ANDREOLI MF; STOKER C; RAMOS J G

Congreso; CONGRESO SAN 2021; 2021

The objective was to explore the importance of neonatal overnutrition on cognition and transcriptional control of steroidogenic enzymes. For that, rats were raised in small litters (3-4 pups/mother; SL), in which pups ingest largeramounts of milk and gain more body weight than rats raised in normal litters (10pups/mother; NL). On PND21, half themale rats were sacrificed (SL vs NL; 15 pups/group) and the rest of maleswere maintained under standard environment (SE) until PND90(SL-SE vs NL-SE; 8rats/group).At PND90, animals were tested inlocomotion activity (LA)and episodic-like memory (ELM).After two weeks, they were sacrificed and brain wasmicrodissected.Using micropunch techniques, dentategirus, CA1 and CA3 regions were isolated formRNA and methylation quantification(results under analysis). At PND21, SL animals had higher body andfat patches weights and greater levels of cholesterol, glucose and triglycerides, than NL rats.However, these metabolic differences were not observed at PND90.No differences were found in LA test although SL-SE rats reported a significantly increased of depositions than NL-SE animals (p