Effects of Mesenchymal Stem Cells on endothelial dysfunction induced by Shiga toxin, the causative agent of Hemolytic Uremic Syndrome.?

DAIANA MARTIRE-GRECCO; ; CARLOS LUZZANI;; LUIS A. CASTILLO;; VERÓNICA A. FURMENTO;; ALEJANDRO LA GRECA;; NAHUEL RODRIGUEZ-RODRIGUES;; VERÓNICA I. LANDONI; ; SANTIAGO G. MIRIUKA; Z; GABRIELA C. FERNÁNDEZ

Cancun

Congreso; XII Congress of the Latin American Association of Immunology - ALAI.; 2018

Introduction: Mesenchymal stem cells (MSC) are multipotent cells that possess known tissue regenerative properties. Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cell (iPS-MSC) has similar characteristics as MSC but can be obtained in larger quantities and more easily. Endothelial tissue damage in the renal glomerulus is one of the most relevant issues that cause kidney failure in Hemolytic Uremic Syndrome (HUS), wich is an endemic pathology in Argentina. Our aim was to investigate if iPS-MSC were able to contribute in the repair of endothelial damage cause by the exposure to lipopolysaccharide (LPS) and Shiga toxin 2 (Stx), the two most important etiological mediators in HUS. Methods: we used an in vitro model of endothelial damage using the cell line HMEC-1 incubated with LPS and/or Stx for 24 h. Results: We found that iPS-MSC exposed to LPS presented a decreased relative migrated area after a wound in the cell monolayer compared to Control cells, but the combination of LPS+Stx reversed this effect (relative area(x102), Control:5,10±0,04; LPS:3,60±0,02*; Stx: 3,80±0,03*;LPS+Stx:6,50±0,04#,*vs. Control and #vs. LPS or Stx, p