PROKARYOTIC RNA MODULATES ENDOTHELIAL-NEUTROPHIL INTERACTIONS

NAHUEL RODRIGUEZ-RODRIGUES; LUIS CASTILLO,; DAIANA MARTIRE-GRECO; FEDERICO BIRNBERG WEISS; VERONICA I LANDONI,; GABRIELA CRISTINA FERNANDEZ

Congreso; Reinion de Biociencias; 2017

Human neutrophils (PMN) are able to sense bacterial viability, ac-tivating and generating a response only against live bacterial stimuli. In this regard, we have demonstrated that prokaryotic RNA (pRNA), present only in live bacteria, is capable of triggering PMN activation and the generation of bactericidal responses. Because endothelial cells can actively regulate the migration of PMN through the endothelium by the expression of adhesion molecules and chemokines, our objective was to determine if pRNA can directly activate the endothelium and modulate the interaction with PMN. In all experiments, a microvascular endothelial cell line (HMEC-1) was stimulated with RNA (1 µg/ml) extracted from E. coli (pRNA) or from human eukaryotic (mononuclear) cells (eRNA), or left untreated, for 24 hs. After stimulation, the expression of ICAM-1 (CD54) in HMEC-1, measured as the MFI by FACS was increased in pRNA-treated cells compared to the other groups (p0.001) . Moreover, the secretion of IL-8 after pRNA exposure was higher (p