CONTRIBUTION OF MESENCHYMAL STEM CELLS TO ENDOTHELIAL REPAIR IN HEMOLITIC UREMIC SYNDROME

DAIANA MARTIRE-GRECO; CARLOS LUZZANI ; ALESSANDRA NORRIS ; VERÓNICA ALEJANDRA FURMENTO ; LUIS ALEJANDRO CASTILLO ; NAHUEL RODRIGUEZ-RODRIGUES; LANDONI VERÓNICA INÈS; SANTIAGO MIRIUKA; GABRIELA CRISTINA FERNÁNDEZ

Congreso; Reinion de Biociencias; 2017

Mesenchymal stem cells (MSC) are multipotent cells that possess known tissue regenerative properties. Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cell (iPS-MSC) has similar characteristics as MSC. Endothelial tissue damage in the renal glomerulus is one of the most relevant issues that cause kidney failure in Hemolytic Uremic Syndrome (HUS). Our aim was to investigate if iPS-MSC were able to contribute in the repair of endothelial damage cause by the exposure to lipopolysaccharide (LPS) and Shiga toxin 2 (Stx), the two most important etiological mediators in HUS. For this purpose, we used an in vitro model of endothelial damage using the cell line HMEC-1 incubated with LPS and/or Stx for 24 h. We found that iPS-MSC exposed to LPS decreased their migratory capacity measured as the migrated area after a wound in the cell monolayer compared to Control cells, but the combination of LPS+Stx reversed this effect (area cm2 (x102 ), Control:5,10±0,04; LPS:3,60±0,02*; Stx: 3,80±0,03*;LPS+Stx:6,50±0,04#,*vs. Control and #vs. LPS or Stx, p