Effect Of Antiinflammatory Drugs On Human Neutrophils (PMN) In Endothelial Cell Damage Induced By Shiga Toxin-1(Stx1).

LANDONI V.I; NEGROTTOS; D ATRI P; RAMOS M.V; FERNANDEZ G.C; PISTONE V; IBARRA C; PALERMO M.S; SCHATTNER M; ISTURIZ M.A

Cordoba Argentina

Congreso; VII Congreso Latinoamericano de Inmunología; 2005

EFFECT OF ANTIINFLAMMATORY DRUGS ON HUMAN NEUTROPHILS (PMN) IN ENDOTHELIAL CELL DAMAGE INDUCED BY SHIGA TOXIN-1(STX1). LANDONI V1; NEGROTTO S1; D´ATRI P1; RAMOS V1; FERNANDEZ G1;PISTONE V2;IBARRA C2; PALERMO M1; SCHATTNER M1; ISTURIZ M1. 1Academia Nacional de Medicina;2 Facultad  Medicina, Universidad Buenos Aires Hemolytic Uremic Syndrome (HUS), characterized by hemolytic anemia, thrombocytopenia and acute renal failure, results in renal endothelial damage and is generally caused by E. coli producing Shiga toxins. We found the maximal activity of Stx when inflammatory agents and neutrophils were present. This may explains the correlation between poor prognosis in HUS and neutrophilia. In this work we studied the effect of antiinflammatory drugs on endothelial cells damage induced by Stx1, inflammatory agents (LPS) and PMN. Human endothelial cells (HUVEC) were preincubated with antiinflammatory drugs and then with LPS and Stx1. Then, PMN treated with antiinflammatory drugs were added. The cytotoxic effect was evaluated by microscopy. Results expressed as percentage of damage of HUVEC treated with LPS, Stx1 and PMN compare  to controls ± SD: dexamethasone: 50±6; N-acetylcysteine: 41±6; ibuprofeno 48±8; methylthiourea: 49±5. Similar results were obtained with renal tubular epithelial cells (HRTEC). The ability of PMN and platelets to bind Stx1 was also evaluated. In conclusion, our results show that antiinflammatory drugs could be used as a tool to reduce the cytotoxicity induced by Stx1.