THE LACK OF NET INDUCTION BY KLEBSIELLA PNEUMONIAE IS NOT REVERSED WITH PLATELETS

BIRNBERG-WEISS; JOSE RAMÓN PITTALUGA; JOSELYN E. CASTRO; LUIS CASTILLO,; DAIANA MARTIRE-GRECCO;; GOMEZ, SONIA A.; LANDONI, VERÓNICA; FERNÁNDEZ, GABRIELA CRISTINA;

Congreso; Sociedad Argentina de Inmunologia; 2022

pneumoniae carbapenemase (Kp)-producing bacteria are associated with significant mortality in immunocompromised patients. We have previously reported that neutrophils (PMN) failed to release neutrophil-extracellular-traps (NET) when challenged with Kp belonging to ST258, whereas Escherichia coli (Eco) was a high NET inducer. It has been reported that PLT activation and PMN:PLT mixed-aggregate formation potrentiate NET induced by LPS and other soluble stimuli. Our aim was to study if the lack of Kp-mediated NET induction can be reversed by the presence of PLT. To assess the effect of both bacteria on PLT activation we measured P-selectin expression by flow cytometry. We found an increment in the percentage of P-selectin-positive PLT when challenged with Kp or Eco (p