INVESTIGADORES
ROSA Mariana Daniela
artículos
Título:
Cadmium induces changes in sucrose partitioning, invertase activities and membrane functionality in roots of rangpur lime (Citrus limonia l. Osbeck)
Autor/es:
PODAZZA, GRISELDA; ROSA, MARIANA; GONZÁLEZ, JUAN ANTONIO; HILAL, MIRNA; PRADO, FERNANDO
Revista:
PLANT BIOLOGY
Editorial:
Thieme
Referencias:
Lugar: Fachzeitschriften. Alemania ; Año: 2006 vol. 8 p. 706 - 714
ISSN:
1435-8603
Resumen:
Abstract: Cadmium (Cd) uptake effects on sucrose content, invertase
activities, and plasma membrane functionality were investigated
in Rangpur lime roots (Citrus limonia L. Osbeck). Cadmium
accumulation was significant in roots but not in shoots
and leaves. Cadmium produced significant reduction in roots
DW and increment in WC. Leaves and shoots did not show significant
differences on both parameters. Sucrose content was
higher in control roots than in Cd-exposed ones. Apoplastic sucrose
content was much higher in Cd-exposed roots than in control
ones. Cd-exposed roots showed a significant decrease in
both cell wall-bound and cytoplasmic (neutral) invertase activities;
while the vacuolar isoform did not show any change. Alterations
in lipid composition and membrane fluidity of Cd-exposed
roots were also observed. In Cd-exposed roots phospholipid
and glycolipid contents decreased about 50%, while sterols
content was reduced about 22%. Proton extrusion was inhibited
by Cd. Lipid peroxidation and proton extrusion inhibition were
also detected by histochemical analysis. This work findings demonstrate
that Cd affects sucrose partitioning and invertase activities
in apoplastic and symplastic regions in Rangpur lime roots
as well as the plasma membrane functionality and H+-ATPase activity.
accumulation was significant in roots but not in shoots
and leaves. Cadmium produced significant reduction in roots
DW and increment in WC. Leaves and shoots did not show significant
differences on both parameters. Sucrose content was
higher in control roots than in Cd-exposed ones. Apoplastic sucrose
content was much higher in Cd-exposed roots than in control
ones. Cd-exposed roots showed a significant decrease in
both cell wall-bound and cytoplasmic (neutral) invertase activities;
while the vacuolar isoform did not show any change. Alterations
in lipid composition and membrane fluidity of Cd-exposed
roots were also observed. In Cd-exposed roots phospholipid
and glycolipid contents decreased about 50%, while sterols
content was reduced about 22%. Proton extrusion was inhibited
by Cd. Lipid peroxidation and proton extrusion inhibition were
also detected by histochemical analysis. This work findings demonstrate
that Cd affects sucrose partitioning and invertase activities
in apoplastic and symplastic regions in Rangpur lime roots
as well as the plasma membrane functionality and H+-ATPase activity.
activities, and plasma membrane functionality were investigated
in Rangpur lime roots (Citrus limonia L. Osbeck). Cadmium
accumulation was significant in roots but not in shoots
and leaves. Cadmium produced significant reduction in roots
DW and increment in WC. Leaves and shoots did not show significant
differences on both parameters. Sucrose content was
higher in control roots than in Cd-exposed ones. Apoplastic sucrose
content was much higher in Cd-exposed roots than in control
ones. Cd-exposed roots showed a significant decrease in
both cell wall-bound and cytoplasmic (neutral) invertase activities;
while the vacuolar isoform did not show any change. Alterations
in lipid composition and membrane fluidity of Cd-exposed
roots were also observed. In Cd-exposed roots phospholipid
and glycolipid contents decreased about 50%, while sterols
content was reduced about 22%. Proton extrusion was inhibited
by Cd. Lipid peroxidation and proton extrusion inhibition were
also detected by histochemical analysis. This work findings demonstrate
that Cd affects sucrose partitioning and invertase activities
in apoplastic and symplastic regions in Rangpur lime roots
as well as the plasma membrane functionality and H+-ATPase activity.
accumulation was significant in roots but not in shoots
and leaves. Cadmium produced significant reduction in roots
DW and increment in WC. Leaves and shoots did not show significant
differences on both parameters. Sucrose content was
higher in control roots than in Cd-exposed ones. Apoplastic sucrose
content was much higher in Cd-exposed roots than in control
ones. Cd-exposed roots showed a significant decrease in
both cell wall-bound and cytoplasmic (neutral) invertase activities;
while the vacuolar isoform did not show any change. Alterations
in lipid composition and membrane fluidity of Cd-exposed
roots were also observed. In Cd-exposed roots phospholipid
and glycolipid contents decreased about 50%, while sterols
content was reduced about 22%. Proton extrusion was inhibited
by Cd. Lipid peroxidation and proton extrusion inhibition were
also detected by histochemical analysis. This work findings demonstrate
that Cd affects sucrose partitioning and invertase activities
in apoplastic and symplastic regions in Rangpur lime roots
as well as the plasma membrane functionality and H+-ATPase activity.
Cadmium (Cd) uptake effects on sucrose content, invertase
activities, and plasma membrane functionality were investigated
in Rangpur lime roots (Citrus limonia L. Osbeck). Cadmium
accumulation was significant in roots but not in shoots
and leaves. Cadmium produced significant reduction in roots
DW and increment in WC. Leaves and shoots did not show significant
differences on both parameters. Sucrose content was
higher in control roots than in Cd-exposed ones. Apoplastic sucrose
content was much higher in Cd-exposed roots than in control
ones. Cd-exposed roots showed a significant decrease in
both cell wall-bound and cytoplasmic (neutral) invertase activities;
while the vacuolar isoform did not show any change. Alterations
in lipid composition and membrane fluidity of Cd-exposed
roots were also observed. In Cd-exposed roots phospholipid
and glycolipid contents decreased about 50%, while sterols
content was reduced about 22%. Proton extrusion was inhibited
by Cd. Lipid peroxidation and proton extrusion inhibition were
also detected by histochemical analysis. This work findings demonstrate
that Cd affects sucrose partitioning and invertase activities
in apoplastic and symplastic regions in Rangpur lime roots
as well as the plasma membrane functionality and H+-ATPase activity.
accumulation was significant in roots but not in shoots
and leaves. Cadmium produced significant reduction in roots
DW and increment in WC. Leaves and shoots did not show significant
differences on both parameters. Sucrose content was
higher in control roots than in Cd-exposed ones. Apoplastic sucrose
content was much higher in Cd-exposed roots than in control
ones. Cd-exposed roots showed a significant decrease in
both cell wall-bound and cytoplasmic (neutral) invertase activities;
while the vacuolar isoform did not show any change. Alterations
in lipid composition and membrane fluidity of Cd-exposed
roots were also observed. In Cd-exposed roots phospholipid
and glycolipid contents decreased about 50%, while sterols
content was reduced about 22%. Proton extrusion was inhibited
by Cd. Lipid peroxidation and proton extrusion inhibition were
also detected by histochemical analysis. This work findings demonstrate
that Cd affects sucrose partitioning and invertase activities
in apoplastic and symplastic regions in Rangpur lime roots
as well as the plasma membrane functionality and H+-ATPase activity.
Citrus limonia L. Osbeck). Cadmium
accumulation was significant in roots but not in shoots
and leaves. Cadmium produced significant reduction in roots
DW and increment in WC. Leaves and shoots did not show significant
differences on both parameters. Sucrose content was
higher in control roots than in Cd-exposed ones. Apoplastic sucrose
content was much higher in Cd-exposed roots than in control
ones. Cd-exposed roots showed a significant decrease in
both cell wall-bound and cytoplasmic (neutral) invertase activities;
while the vacuolar isoform did not show any change. Alterations
in lipid composition and membrane fluidity of Cd-exposed
roots were also observed. In Cd-exposed roots phospholipid
and glycolipid contents decreased about 50%, while sterols
content was reduced about 22%. Proton extrusion was inhibited
by Cd. Lipid peroxidation and proton extrusion inhibition were
also detected by histochemical analysis. This work findings demonstrate
that Cd affects sucrose partitioning and invertase activities
in apoplastic and symplastic regions in Rangpur lime roots
as well as the plasma membrane functionality and H+-ATPase activity.+-ATPase activity.