CONICET | Buscador de Institutos y Recursos Humanos

Althoughglyphosate-based herbicides are considered safe due to their low persistence,new evidence suggests that they could affect the correct embryo implantationand development even in low doses. The trophectoderm surrounding the blastocystplays a pivotal role in the invasion, migration, and spiral arteries remodelingfrom the decidua. This process is high-regulated, and its alterations couldcarry out preeclampsia, miscarriages, and other associated pathologies.Previous studies demonstrated that concentrations of 0.2 and 2 μM of glyphosate (G) stimulatedmigration activity in a human endometrial carcinoma cell line (Ishikawa). Thisstudy aims to analyze ex vivo the effect of 2.5 μM G in murine blastocystdevelopment. The cellular migration was also assayed using the trophoblast cellline HTR-8/SVneo with 0.625, 1.25, 2.5, 5, and 10 μM of G. E3.5 embryos, recovered from pregnantBALC/b mice, were placed on murine uterine epithelial cells monolayer with 2.5 μM of G or vehicle (V). Theimplantation area and hatching/attachment time were registered for sixconsecutive days. The wound healing assay was performed to evaluate themigration activity. The monolayer was pretreated with G concentrations for 24h, and the medium was renewed after scratching. Then, the uncovered areas wereregistered at 0 and 12 h. Cell viability was determined spectrophotometricallyafter 24 and 48 h of treatment using WST-1 reagent and by counting cells in ahemocytometer. All the assays were performed in triplicate. The blastocystimplantation area (G: 0.47 ± 0.03 mm2; V: 0.32 ± 0.14 mm2) andhatching/attachment time (G: 42.3 ± 10.5 h; V: 45.2 ± 19.9 h) were similarbetween groups. Cellular migration was stimulated at 0.625 μM G compared to V (P  0.05). These results suggest thateven low concentrations of G could dysregulate some processes associated withimplantation.

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