Searching for potential targets of benzophenone-3 action involved in mammary gland alveolar growth and function

SCHIERANO-MAROTTI G; ALTAMIRANO GA; GOMEZ AL; ODDI SL; MUÑOZ-DE-TORO M; KASS L

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

The UV-filter benzophenone-3(BP3), commonly used in sunscreens, has been shown to alter the mammary glanddevelopment. Previously, we have shown that intrauterine exposure to BP3 altersthe histoarquitecture of the lactating murine mammary gland by modifying thealveolar area, alveolar size ratio and the myoepithelial linear density,without changing the expression of milk proteins. Potential targets of BP3action involved in alveolar growth and function could be signal transducer andactivator of transcription 5 (STAT5a), Ets transcription factor 5 (ELF5) andglucocorticoid receptor (GR). Therefore, our aim was to evaluate the expressionof these molecules in the lactating mammary gland of the F1 female offspringafter the intrauterine exposure to BP3. Pregnant F0 C57BL/6 mice were dermallyexposed to vehicle (sesame oil; control) or 50mg BP3/kg/day (50BP3) fromgestation day 8.5 to 18.5. At 8 weeks old, F1 female offspring were bred, andmammary gland samples were obtained on lactation day 10. The mRNA expression ofSTAT5a, ELF5 and GR was evaluated by real-time RT-PCR. The 50BP3 group showedsimilar expression of STAT5a, ELF5 and GR to the control group (p > 0.05).In conclusion, STAT5a, ELF5 and GR do not seem to be involved in the alveolar modificationsinduced by intrauterine exposure to BP3 in the lactating murine mammary gland.