Effects of exposure to benzophenone-3 on early molecular markers of mammary involution in HC11 cells

GOMEZ AL; BECKLEY MB; ALTAMIRANO GA; ODDI SL; SCHIERANO-MAROTTI G; MORALES-RUIZ T; MARTINEZ-MACÍAS MI ; MUÑOZ-DE-TORO M; ROLDÁN-ARJONA T; KASS L

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

Mammary involution is a period of intense tissue remodeling,and its alteration may result in inadequate lactation or predispose to tumor development.In this sense, the UV-filter benzophenone-3 (BP3) has been shown to haveestrogenic activity and to alter apoptosis, which could affect this process. Here,we investigated whether exposure to BP3 modifies the expression of earlymolecular markers of involution in a murine mammary cell line. HC11 cells were grownto confluence and exposed to vehicle (0.1% ethanol) and 1 or 1000 nM of BP3 for72 h. The cells were then differentiated with lactogenic hormones for another 72h, after which the hormones were withdrawn. Cell viability was assessed immediately after BP3exposure. Beta-casein (CSN2), Stat3, Bcl2 and Bax mRNAexpression was analyzed at 0, 24, 48 and 72 h, whereas DNA methylation of Bcl2and Bax promoters was assessed by bisulfite pyrosequencing at 24 h afterremoval of the lactogenic hormones. Cell viability and Stat3 mRNA expression werenot affected by BP3 exposure. The exposure to BP3 1 nM increased the mRNAexpression of CSN2 (11.9-fold), Bcl2 (5.3-fold) and Bax (1.5-fold) at 24 h, andreduced CSN2 expression (9.8-fold) at 72 h compared to vehicle. Bcl2 promoter showedreduced DNA methylation at 24 h in cells exposed to BP3 1 nM compared tovehicle, and no differences were observed in the Bax promoter. Our results showedthat BP3 exposure could interfere with the normal involution of the mammarygland and modify the expression of key molecular markers of this process.