METFORMIN TREATMENT MODULATES MACROPHAGE RESPONSE AGAINST T. CRUZI INFECTION.

BAIGORRÍ, RE; ANA, Y; BRUGO, MB; VIANO, ME; RODRIGUEZ GALAN, MC; MOTRAN, C; STEMPIN, C; CERBAN, F

Congreso; Reunión de Sociedades de Biociencias 2021 - LXIX REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA; 2021

In acute phase of T. cruzi infection, both innate and adaptive immunity are necessary to control parasite replication. Macrophage (Mf) and T cells orchestrate the inflammatory response that controls parasite burden. However, an exacerbated immune response results in tissue damage, mainly by ROS and RNS release. Metformin (Mf), a type 2 diabetes drug, reduces inflammation in models of aging and pollution. In our in vivo model of T. cruzi infection in Balb/c mice, we observed that peritoneal and spleen Mf increase iNOS expression during acute phase. Pretreatment of BMDM with Mf prevents intracellular parasite replication and promotes proinflammatory cytokine production. We also infected RAW cells and then were treated with PBS or Mf 1mM. This treatment decreased ROS production (p