Isolation and selection of endophytic sporeforming Bacteria with plant growth promoting Properties isolated from ilex paraguariensis st. Hil. (yerba mate)

LACZESKI,MARGARITA E.; ONETTO, ANDREA L.; CORTESE, JULIETA; MALLOZZI, GISELLA Y; CASTRILLO, MARIA LORENA; BICH, GUSTAVO; GORTARI, FERMÍN; ZAPATA,PEDRO D; OTEGUI, MONICA B

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS.

ACAD BRASILEIRA DE CIENCIAS

Lugar: Rio de Janeiro; Año: 2019

0001-3765

The inadequate management of yerba mate (Ilex paraguariensis St. Hil.) plantations has derived in a diminish in their production levels. A new approach to enhance productivity could be the use of microorganisms that enhance plant growth. The objective of this study was to isolate spore-forming endophytic bacteria with plant growth promoting properties associated with yerba mate seedlings and determine their effect under controlled laboratory conditions. Isolates were selected based on their sporulation capacity and evaluated for in vitro plant growth promoting properties (nitrogen fixation in nitrogen free medium, phosphates solubilization using NBRIP medium, production of siderophores using Chrome Azurol S agar plates and synthesis of indolic compounds using Salkowski?s reagent). Yerba mate seedlings were inoculated with the most promising isolates (12RS3, 19RS3 and T5S-T4). After 120 days plants showed higher root dry weight when inoculated with isolate 19RS3 and higher aerial part dry weight with 19RS3 and T5S-T4 (identified via analyses of the sequence of their 16S rDNA gene as Bacillus circulans and Bacillus altitudinis, respectively). These isolates will be subjected to further studies, seeking to develop a biofertilizing product designed for yerba mate and adapted to the climatic conditions, soils and microbiome of the areas where it is cultivated