Utilidad de la enzima catalasa como indicador de actividad en la nefropatía lúpica

BALBI, BÁRBARA; OCAMPO ALZATE, CARLOS DAVID; BASIGLIO CL; PEZZARINI, ELEONORA; SVETAZ MJ; BEARZOTTI M; BOTTAI, HEBE; DANIELE, STELLA; ROSSI, MARÍA FLORENCIA; TAURIZANO, NATALIA; RODENAS, MARTÍN; MONJE, ADRIANA; PELUSA HF; ARRIAGA SMM

Rosario

Congreso; XVII Congreso y XXXV Reunión Anual de la Sociedad de Biología de Rosario (SBR); 2016

Sociedad de Biología de Rosario

Lupus nephropathy (LN), occurring in 60% of patients with systemic lupus erythematosus (SLE), is a clinical complication of poor prognosis. Despite advances in the treatment of LN, 10-15% patients progress to end stage renal failure. Early diagnosis and treatment, however, can significantly improve long term prognosis. Previously, we found that oxidative stress (OS) is increased in patients with active LN (ALN); a pro-oxidant status prevails and antioxidant defenses would not be sufficient to mitigate it. Our aim was to analyze if any of the following OS parameters, tiobarbituric acid-reactive substances (TBARS), superoxide dismutase (SOD) and catalase (CAT), is a better marker of activity in LN. We studied patients with dignosis of LN and, as a control group, patients with diagnosis of inactive LN (ILN) from Nephrology Service of Hospital Provincial del Centenario. We determined: a) TBARS levels, by a colorimetric technique (n=24 for both groups), b) SOD activity, with a commercial kit (Randox Ltd.) (n=17 for ALN; n=24 for ILN) and c) CAT activity, following H2O2 decomposition at 240 nm (n=13 for ALN; n=11 for ILN). Results obtained for TBARS [pmol malonyldialdehyde/ml; median (range)], SOD (U/gHb; mean±SD) and CAT (U/gHb; mean±SD) in ALN vs ILN were respectively: 40 (0-172) vs 17 (0-420); 1114±710 vs 1069±541 y 1.14±0.75 vs 0.5±0.2. No significant differences between groups were found for TBARS (p=0.25) nor for SOD (p=0.81) but for CAT (p=0.01). We conclude that the activity of CAT would be useful to discriminate between patients with ALN and ILN. Then, determination of CAT, which simple and low cost, could then be used as an additional predictor of activity in renal disease.