IMPAIRMENT OF ENDOGENOUS BILIRUBIN GENERATION AGGRAVATES BILE-DUCT LIGATION INJURY IN RATS: ROLE OF BILIRUBIN ANTIOXIDANT EFFECTS

MARTÍN, PL; RAZORI, MV; CORBO, ML; HARVEY GB; SANCHEZ POZZI, EJ; ROMA, MG; BASIGLIO, CL

Mar del Plata

Congreso; Reunión Conjunta Sociedades de Biociencias; 2022

SAFIS

We showed that hemeoxygenase-1 (HO1) induction, and consequent bilirubin (BR) elevation, protects the liver from oxidative stress (OS)-induced cholestasis in vivo. Here, we evaluated the effect of HO1 inhibition on the endogenous levels of BR and its protective action in obstructive cholestasis. Male Wistar rats were subjected to 7-day bile-duct ligation (BDL, n=7) or sham surgery (Sh, n=5). HO1 was inhibited with Zn(II) protoporphyrin IX (PP, 25 mg/Kg b.w., i.p.), 24h before BDL (PP+BDL, n=10) or Sh (PP+Sh, n=6). Results are expressed as media±SD and analyzed by Kruskal-Wallis’ test. After BDL, plasma BR levels (mg/dl) were higher in BDL (8.46±1.70a) than in PP+BDL (3.90±2.80b), confirming HO1 inhibition (ap