INVESTIGADORES
WELCHEN Elina
artículos
Título:
Delta subclass HD-Zip proteins and a B-3 AP2/ERF transcription factor interact with promoter elements required for expression of the Arabidopsis cytochrome c oxidase 5b-1 gene.
Autor/es:
COMELLI, RAUL; WELCHEN, ELINA; KIM HJ.; HONG, JC. ; GONZALEZ, DANIEL H.
Revista:
PLANT MOLECULAR BIOLOGY
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2012 p. 157 - 167
ISSN:
0167-4412
Resumen:
We have identified transcription factors that
interact with a promoter region involved in expression of
the Arabidopsis thaliana COX5b-1 gene, which encodes an
isoform of the cytochrome c oxidase zinc binding subunit.
Elements with the core sequence ATCATT, involved in
induction by sugars, are recognized both in vitro and in
one-hybrid assays in yeast by HD-Zip proteins from
the delta subclass and, though less efficiently, by the trihelix
transcription factor GT-3b. DistalB-like elements
(CCACTTG), required for induction by abscisic acid
(ABA), interact with ESE1, a member of the B-3 subgroup
of AP2/ERF transcription factors. The HD-Zip protein
Athb-21 and ESE1 are able to interact in yeast two-hybrid
assays with the ABA responsive element binding factor
AREB2/ABF4, which binds to a G-box absolutely required
for expression of the COX5b-1 gene. Overexpression of the
identified transcription factors in plants produces an
increase in COX5b-1 transcript levels. Moreover, these
factors are able to induce the expression of a reporter gene
located in plants under the control of the relevant COX5b-1
promoter regions required for expression. Analysis of
promoter regions of COX5b genes from different plant
species suggests that the identified transcription factors
were recruited for the regulation of COX5b gene expression
at different stages during the evolution of dicot plants.