Sparse labelling with AAV-PHP.eB, a noninvasive gene delivery method: Optimization of a protocol for morphological and anatomical connectivity analyses.

PRETELL ANNAN CA.,; TRIBBIA LT.; LA IACONA G,; RELA L.; TARAVINI IRE.; PAFUNDO DE.; BELFORTE JE.

Montevideo

Encuentro; 1st Latin America Bioimaging Meeting; 2022

•Research into the physiological and pathophysiological processes concerning the central nervous system (CNS) fundamentally relies in analyses of structure-function relationships at the single cell level and studies of anatomical connectivity at the neural network level.•Cellular morphological studies are better performed when a sufficiently sparse labelling of whole arborizations is obtained. However, methods such as Golgi staining can impede further neurochemical analyses.•For studies of neuroanatomical connectivity, conventional or viral tracers are usually used. Nevertheless, it can prove difficult to obtain thorough information regarding the morphology of the cellular elements receiving these projections.•In vivo noninvasive sparse fluorescent labeling can be adequately combined with fluorescenttracers and immunofluorescent stainings for a comprehensive study of morphology, connectivity and neurochemistry.