Citric acid coated magnetic nanoparticles for drug targeting: characterization and biocompatibility on vascular and bone systems

ADRIAN CAMPELO; MARIELA A. AGOTEGARAY; SABRINA CEPEDA; MARISA SANDOVAL; VERÓNICA L. LASSALLE; VIRGINIA MASSHEIMER

Buenos Aires

Congreso; XXXVI Reunión Anual de la Asociación Argentina de Osteología y Metabolismo Mineral; 2019

Introduction: Citric acid coated magnetic nanoparticles (ca-MNP) consist on a magnetite core coated with citric acid molecules. By the application of an external magnetic field, these MNP can be directed to a specific site of the organism through the vascular system. This nanotechnology find promising applications in targeted drug delivery. The citric acid coating confers biocompatibility and acts as platform for the incorporation of biomolecules. Looking forward bone targeting of caMNP, the present work aims to evaluate their vascular and bone biocompatibility of these MNP. Results: The caMNP were obtained by the co-precipitation method employing ferrous and ferric salts in the presence of citric acid. They showed to have a diameter of 260±14nm and Z potential of -25.7±4.3mV, being these physicochemical properties suitable for biomedical applications.Primary cultures of vascular endothelial cells (EC) and osteoblasts (OB) were obtained by explant technique from aorta and calvaria of Wistar rats respectively. Cells were exposed to 1, 10 and 100μg/ml of MNPs for 48h and specific cellular processes were evaluated.Cell viability (MTT assay) was assayed in EC and OB. No effect of caMNP was observed below 10μg/ml. 100μg/ml of MNPs induced a 22% and 16% decrease in EC and OB viability respectively (p