Hemocompatibility study of amorphous silica nanoparticles

TAMARA RODRÍGUEZ ÁLVAREZ; MARIELA AGOTEGARAY

Congreso; Reunión Anual de Sociedades de Biociencia: SAIC, SAI, AAFE, Nanomed-Ar; 2021

The study of potential toxicity of amorphous silica nanoparticles (SiNPs) has been assessed in human blood to better understand their usefulness as drug carriers, in special for hematological disorders. Size represents a crucial parameter in terms of internalization and disruption of blood cells. In this work, SiNPs were synthesized by Stöber method with modifications, varying the amounts of water and ethanol; temperature and time of reaction as variables determinant of size. Besides, SiNPs were modified by treatment with NaOH to generate pores in the surface for future pharmacological prospects. The SiNPs obtained were characterized by FTIR spectroscopy to evaluate qualitative composition; by DLS to determine hydrodynamic diameter (Hd) and zeta potential to infer surface charge. Hemolytic activity, LDH levels and oxidative stress were studied. Exposure to SiNPs was conducted by mixing NPs dispersed in isotonic saline solution of NaCl 0.9% at concentrations of 1000, 500, 250, 100 and 50 µg/mL with healthy human blood, during 1h. Positive controls were processed. Free hemoglobin was determined by UV spectroscopy. LDH levels were studied employing a commercial kit. Evaluation of oxidative stress was conducted by TBARS determination. FTIR spectroscopy confirmed SiO2 as main component of NPs. All formulations obtained resulted monodisperse with Hd near 200nm with negative surface charge. Results have not evidenced changes in erythrocytes lysis in comparison to control (p