INVESTIGADORES
MENDOZA ZELIS Pedro
congresos y reuniones científicas
Título:
Synthesis and cell internalization of magnetite nanoparticles for magnetic hyperthermia applications
Autor/es:
M. E. DE SOUSA; P. GIRARDIN; M. B. FERNÁNDEZ VAN RAAP; G. A. PASQUEVICH; P. MENDOZA ZÉLIS; I. J. BRUVERA; F. H. SÁNCHEZ; R. G. GOYA; Y. E. SOSA
Lugar:
La Plata
Reunión:
Conferencia; HK 2010 - Humboldt Kolleg; 2011
Institución organizadora:
Physics Department - Faculty of Exact Sciences National University of La Plata
Resumen:
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-Magnetic
hyperthermia treatment appears as a viable alternative or
complementary procedure for localized cancerous tumors therapy. The
treatment consists of locally injecting magnetic nanoparticles (MNP)
into the tumor and exposing it to an oscillating magnetic field in
the radio frequency range. When exposed to this field, the MNP
dissipates energy causing a temperature increase in the zone that
contains nanoparticles, inducing tumor cell death by apoptosis at or
above 42
°C. MNP energy dissipation is characterized by the specific power
loss parameter (SLP) which expresses the power absorbed by the MNP
from the field per mass unit of MNP.
13
nm size magnetite nanoparticles with
saturation magnetization of 60 emu/g, were synthesized by
co-precipitation route using iron salts and a strong base, and
finally set into aqueous suspension. Their physical properties were
characterized with various techniques and suggest their adequacy for
hyperthermia treatment and cell internalization requirements.
Measured SPL value for this solution, using a coil fed with a signal
of 260 kHz and amplitude of 500 Oe (39.8 kA m-1),
was 203 W g-1.
Internalization
methodology for anionic magnetic
nanoparticles, which is achieved, for instance, by adsorption of
citrate anions to the ferric oxide surface, is known to be well
established in various tumoural and normal cells. Here, bare MNP
nanoparticle were internalizated in A549 cells from human lung
adenocarcinoma for two NP concentrations of 20 and 50 µg/ml. MNP
incorporation into cells follows the endocytic pathway. It is
observed that MNP concentration inside cells is larger and more
densely packed for the larger MNP concentration. Confocal, atomic
force and transmission electron microscopies were used to image the
in vitro uptake of nanoparticles by A549 cells.