Efficacy of thymol against Echinococcus granulosus protoscoleces

ELISSONDO MARIA CELINA; ALBANI CLARA MARÍA; GENDE LIESEL; EGUARAS MARTIN; DENEGRI GUILLERMO MARÍA

Parasitology International

Elsevier

Lugar: Irlanda; Año: 2008 vol. 57 p. 185 - 190

1383-5769

  The aim of the present work was to determine the in vitro protoscolicidal effect of thymol against Echinococcus granulosus. Protoscoleces ofin vitro protoscolicidal effect of thymol against Echinococcus granulosus. Protoscoleces of  14 E. granulosus were incubated with thymol at concentrations of 10, 5 and 1 ìg/ml. The first signs of thymol-induced damage were observed E. granulosus were incubated with thymol at concentrations of 10, 5 and 1 ìg/ml. The first signs of thymol-induced damage were observed 15 between 1 and 4 days post-incubation. The maximum protoscolicidal effect was found with thymol at 10 ìg/ml, viability reduced to 53.5±11.9%between 1 and 4 days post-incubation. The maximum protoscolicidal effect was found with thymol at 10 ìg/ml, viability reduced to 53.5±11.9% 16 after 12 days of incubation. At day 42, viability was 11.5±15.3% and, reached 0% after 80 days. Thymol at concentrations of 5 and 1 ìg/mlafter 12 days of incubation. At day 42, viability was 11.5±15.3% and, reached 0% after 80 days. Thymol at concentrations of 5 and 1 ìg/ml 17 provoked a later protoscolicidal effect. Results of viability tests were consistent with the tissue damage observed at the ultrastructural level. Theprovoked a later protoscolicidal effect. Results of viability tests were consistent with the tissue damage observed at the ultrastructural level. The 18 primary site of damage was the tegument of the parasite. The morphological changes included contraction of the soma region, formation of blebsprimary site of damage was the tegument of the parasite. The morphological changes included contraction of the soma region, formation of blebs 19 on the tegument, rostellar disorganization, loss of hooks and destruction of microtriches. The data reported in this article demonstrate a clear inon the tegument, rostellar disorganization, loss of hooks and destruction of microtriches. The data reported in this article demonstrate a clear in 20 vitro effect of thymol against E. granulosus protoscoleces vitro effect of thymol against E. granulosus protoscoleces