INVESTIGADORES
LASCANO Hernan Ramiro
artículos
Título:
Superoxide Dismutase and Glutathione Reductase overexpression in wheat protoplast: changes in cellular redox state and photooxidative stress tolerance
Autor/es:
MARIANA MELCHIORRE; GERMÁN ROBERT; VICTORIO TRIPPI; ROBERTO RACCA; RAMIRO LASCANO
Revista:
PLANT GROWTH REGULATION
Editorial:
Springer
Referencias:
Año: 2009 vol. 57 p. 57 - 68
ISSN:
0167-6903
Resumen:
Abstract: In previous works, we have established a correlation between antioxidant system response and
tolerance to drought, osmotic stress and photooxidative stress of different wheat cultivars with contrasting
drought tolerance. In the present work, a protocol to obtain and transform wheat protoplasts was established.
Transgenic protoplasts with Manganese Superoxide Dismutase (Mn-SOD) (E.C.: 1.15.1.1) and Glutathione
Reductase (GR) (E.C.: 1.6.4.2) overexpression in chloroplasts were obtained, and their responses to
photooxidative stress were characterized. Protoplasts with Mn-SOD or GR overexpression, showed different
responses and tolerance to photooxidative stress. Protoplasts with Mn-SOD overexpression showed lower
levels of oxidative damage, higher level of endogenous hydrogen peroxide and a great induction of total
SOD and GR activities during photooxidative treatments. In protoplasts with GR overexpression the
oxidative damage provoked by the photooxidative treatment was similar to control protoplasts, the GSH
content and GSH/GSH+GSSG ratio were higher than control and Mn-SOD transformed protoplast, and total
SOD and GR activities were not induced. Our results suggest that the differential responses and tolerance to
photooxidative stress given by Mn-SOD or GR overexpression, also depend on the effects of these enzyme
activities over the cellular redox state balance, which modulate the responses to photooxidative stress