BIOACTIVE SCAFFOLDS WITH THE CAPABILITY TO RELEASE MULTIPLE BIOACTIVE AGENTS APPLIED IN TISSUE

R. VIDOTTO; K. ZHENG; J. P. CATTALINI; A. R. BOCCACCINI; V. MOURIÑO

DARMSTADT

Conferencia; Materials Science and Engineering (MSE-Congress) 2018; 2018

IIntroductionThe possibility of adding different functionalities to scaffolds for bone regeneration allows the incorporation of new properties to guide and promote the formation of new bone [1, 2]. In this regard, an interesting strategy for bone regeneration is to use the scaffold as a controlled administration system for different agents that play an important role in osteogenesis, and / or prophylaxis against infections, to promote integration between biomaterials and the tissue to be regenerated [3-5]. Metal ions such as calcium (Ca2*) and copper (Cu2*) stimulate the promotion of bone formation and regeneration and its vascularization [6]. In addition, Cu2* and Zinc (Zn2*) have bacteriostatic properties associated with low human toxicity and bacterial resistance [6, 7]. In this study, a new nanocomposite and bioactive scaffold was developed that exhibits the capacity to supply multiple therapeutic ions based on bioglass nanoparticles partially replaced with copper and alginate ions crosslinked with Ca2* and Zn2*.Results and DiscussionThe resulting composite scaffolds exhibited homogeneous structures, adequate porosity and sufficient mechanical strength when purchased with previous reports1. In addition, the bioactive nature of the scaffolds was confirmed due to the growth of hydroxyapatite crystals on the surface after 14 days in simulatedbody fluid. The degradation study indicated that the scaffolds crosslinked with Zn 2 + and Ca2 + (in that order) appear to be stable over time showing a slow degradation rate. According to the analysis of the release profile of Cu2 + (from the bioactive glass nanoparticles) and Ca2 + and Zn3 + (from the crosslinked polymer), the values of the released amount are within the ranges reported for promote angiogenesis, osteogenesis and inhibition of bacterial proliferation, respectively.ConclusionsThe present study represents a positive advance in the preparation of therapeutic scaffolds with the capacity to release in a sequential and prolonged multiple bioactive agents for applications in regenerative medicine. Further studies are required to characterize the cellular response to scaffolds and their ability to form bone both in vitro and in vivo.References1. C. Wu, Y. Zhou, M. Xu, P. Han, L. Chen, J. Chang, Y. Xiao. Biomaterials 2013, 34, 422-33.2. R. Ravichandran, S. Gandhi, D. Sundaramurthi, S. Sethuraman, U. Krishnan. J. BiomaterSciPolym 2013, 24, 1988-2005.3. G. Harris, K. Rutledge, Q. Cheng, J. Blanchette, E. Jabbarzadeh. Curr Pharm Des 2013, 19, 3456-65.4. V. Mouriño, J. Cattalini, J. Roether, P. Dubey, I. Roy, A. R. Boccaccini. Expert Opin Drug Deliv 2013, 10, 1353-65.5. V. Mouriño, A. R. Boccaccini, J R Soc Interface 2010, 7, 209- 27.6. V. Mouriño, J. Cattalini, A. R. Boccaccini. J R Soc Interface 2012, 9, 401-19.7. V. Mouriño, P. Newbyand A. R. Boccaccini, Adv. Eng. Mater. 2010, 12: B283?B291.