Assesment of nanosized bioactive glass/collagen composites against staphylococcus aureus cells viability

J. RIVANDENEIRA; V. CADENA; V. MOURIÑO; A.R. BOCCACCINI; M.C. AUDISIO; A. GORUSTOVICH

Mar del Plata

Taller; 2° taller de órganos artificiales, biomateriales e ingeniería de tejidos; 2011

The aim of this study was to assess the possible anti-bacterial effects of new composite materials developed from collagen and inorganic bioactive glass nano-particles. For the first time for this end, we employed coating techniques based on stable colloidal suspensions of nano-particles (20-60nm) of bioactive glass (n-BG) from the SiO2-CaO-Na2O-P2O5 (45S5) system on type I bovine collagen membranes, as well as incorporating different weight percentages of n-BG to the collagen gel for the subsequent elaboration of  membranes. The materials obtained were characterized by SEM-EDS. Anti-bacterial assays were carried out with S. aureus (ATCC 29213), because of the importance of this pathogen in failures associated with biomaterial implants. Bacterial suspensions of S. aureus (1x104 a 1x106 cfu/mL) were prepared and then put in contact with the biomaterials at 37°C. After 0, 2, 3, 24 and 48h of incubation, their viability was determined by counting in Mueller-Hinton agar plates. Plates were incubated at 37°C for 48h in order to count colonies. The membranes were prepared for observation under SEM. Our results revealed the presence of bacterial cells on the surface of the collagen membranes with or without coating and/or incorporation of 45S5 n-BG. Furthermore, counting in plates did not show significant inhibition of the S. aureus strain analyzed.