Novel Cu2+ releasing composite films for bone tissue engineering

J.P. CATTALINI; L. HARO; A. GORUSTOVICH; A.R. BOCCACCINI; S.E. LUCANGIOLI; V. MOURIÑO

Taller; 2° taller de órganos artificiales, biomateriales e ingeniería de tejidos; 2011

Biodegradable and bioactive materials with sufficient structural integrity and angiogenic properties based on organic polymer-nanobioactive glass composite films were developed. Films were loaded with Cu2+. Morphology features were observed by SEM and the presence of Cu2+ was confirmed by EDX. Films bioactivity was analysed through the growing of hydroxyapatite on the surface of the films, which was investigated by XRD. In vitro release of Cu2+ was quantified by UV method. Cellular in vitro studies were carried out to assess the ability of these films to support the proliferation of human umbilical vein endothelial cells (HUVECs). The incorporation of bioactive glass nanoparticles (NBG) into the films significantly improved their mechanical properties when compared with films without NBG. In addition, the novel composite films induced an angiogenic effect in vitro promoting the proliferation of HUVECs, due to the presence of Cu2+. Biomineralization studies confirmed the deposition of hydroxyapatite on the surface of the films, indicating their bioactive nature. Thus, it was shown that the novel composite films possess relevant physicochemical and biological effects, which make these materials promising candidates for bone tissue engineering applications. Biodegradable and bioactive materials with sufficient structural integrity and angiogenic properties based on organic polymer-nanobioactive glass composite films were developed. Films were loaded with Cu2+. Morphology features were observed by SEM and the presence of Cu2+ was confirmed by EDX. Films bioactivity was analysed through the growing of hydroxyapatite on the surface of the films, which was investigated by XRD. In vitro release of Cu2+ was quantified by UV method. Cellular in vitro studies were carried out to assess the ability of these films to support the proliferation of human umbilical vein endothelial cells (HUVECs). The incorporation of bioactive glass nanoparticles (NBG) into the films significantly improved their mechanical properties when compared with films without NBG. In addition, the novel composite films induced an angiogenic effect in vitro promoting the proliferation of HUVECs, due to the presence of Cu2+. Biomineralization studies confirmed the deposition of hydroxyapatite on the surface of the films, indicating their bioactive nature. Thus, it was shown that the novel composite films possess relevant physicochemical and biological effects, which make these materials promising candidates for bone tissue engineering applications.