Determination of copper in composite biomaterials by capillary electrophoresis: an UV-direct method based on in situ complex formation

J. P. CATTALINI; V MOURIÑO; S. LUCANGIOLI

Analytical Methods

Royal Society of Chemistry

Año: 2016

1759-9660

This work describes the optimization and validation of an UV-direct capillary electrophoresis method for theanalysis of copper ions in samples obtained from release studies of bone-tissue-engineering compositebiomaterials. Ethylenediaminetetraacetic acid was used as a complexing agent added to the backgroundelectrolyte to modulate the selectivity of the separation and quantitation of free copper ions. Theseparations were performed under reverse polarity and the parameters of validation such as specificity,linearity, limits of detection and quantitation, precision, accuracy and robustness were evaluated. Themethod resulted to be suitable for copper determination in biomaterials and showed advantages such asrapidness, specificity and suitable sensibility, achieving a limit of detection and quantitation of 0.05 and0.16 mg mL1, respectively.