Electrophoretic Deposition of Gentamicin-Loaded Bioactive Glass/ 2 Chitosan Composite Coatings for Orthopaedic Implants

F. PISHBIN; V. MOURIÑO; S. FLOR; S. KREPPEL; V. SALIH; M. RYAN; A. R. BOCCACCINI

ACS APPLIED MATERIALS & INTERFACES

AMER CHEMICAL SOC

Lugar: Washington ; Año: 2014 vol. 6 p. 8796 - 8806

1944-8244

Despite their widespread application, metallic orthopaedic prosthesis failure still occurs because of lack of adequate bone-bonding and the incidence of post-surgery infections. The goal of this research was to develop multifunctional composite chitosan/Bioglass coatings loaded with gentamicin antibiotic as a suitable strategy to improve the surface properties of metallic implants. Electrophoretic deposition (EPD) was applied as a single-step technology to simultaneously deposit the biopolymer, bioactive glass particles, and the antibiotic on stainless steel substrate. The microstructure and composition of the coatings were characterized using SEM/EDX, XRD, FTIR, and TGA/DSC, respectively. The in vitro bioactivity of the coatings was demonstrated by formation of hydroxyapatite after immersion in simulated body fluid (SBF) in a short period of 2 days. High-performance liquid chromatography (HPLC) measurements indicated the release of 40% of the loaded gentamicin in phosphate buffered saline (PBS) within the first 5 days. The developed composite coating supported attachment and proliferation of MG-63 cells up to 10 days. Moreover, disc diffusion test showed improved bactericidal effect of gentamicin-loaded composite coatings against S. aureus compared to control non-gentamicin-loaded coatings.