C-terminal-truncated and full length hemeoxygenase-1 exert opposite behavior of head and neck cancer cells.

MASCARÓ, M.; ALONSO, E.G.; GANDINI, N.A.; FERRONATO, M.J.; GUEVARA, J.A.; ALONSO, E.N.; COLÓ, G.P.; PICHEL, P.; RECIO, S.; CURINO, A.C.; FACCHINETTI, M.M.

Mar del Plata

Congreso; LXIV Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2019

Sociedad Argentina de Investigaciones clínicas

We previously reported that heme oxygenase-1 (HO-1) protein is up-regulated in human HNSCC samples and that it is localized in the cytoplasmic and nuclear compartments. We also reported that high expression of HO-1 mRNA is associated with worst survival and that pharmacological activation of HO-1 by hemin increases viability of HN13 cells. However, how full length (FL-HO1) and C-terminal truncated (t-HO1) HO-1 affects HNSCC remains elusive. In this study, we aim to elucidate if such forms of HO-1 impacts on head and neck cancer cells behavior. We established the FL-HO1 and t-HO1 overexpressing HN13 cells. We evaluated cell viability by crystal violet method, cell cycle progression by propidium iodide staining and flow cytometry, and cell migration by wound healing assay. In addition to our previous results using hemin, we found that 80uM hemin increased cell number in S- (p