Antitumoral and antimetastatic activity of Maitake D-Fraction in triple-negative breast cancer cells.

ALONSO, E.N.; FERRONATO, M.J.; GANDINI, N.A.; FERMENTO, M.E.; GUEVARA, J.A.; MARIANI, F.; FACCHINETTI, M.M.; CURINO, A.C.

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias.; 2017

D-Fraction is a proteoglucan extracted from Grifola frondosa (Maitake) mushroom. Previously, we reported that D-Fraction decreases breast cancer (BC) cell viability regardless of hormone receptors and HER2 status of cells. Furthermore, D-Fraction reduces tumor burden and lung metastases in a murine model with hormone-independent LM3 cells. In triple-negative (TNBC) MDA-MB-231 cells, we also demonstrated that D-Fraction decreases their migration and invasion capacity. The purpose of the current study is to identify the cellular and molecular mechanisms by which D-Fraction decreases the migratory/invasive potential of MDA-MB-231 cells. In addition, we propose to evaluate the antitumoral effect of D-Fraction in 4T1 cells, another cell line representative of TNBC subtype. By western blot, we found that D-Fraction increases E-cadherin expression in MDA-MB-231 cells compared to vehicle treatment (p