Antitumoral effects of Grifola frondosa (Maitake) D Fraction in breast cancer.

CURINO, A.C.; FERRONATO, M.J; GANDINI, N.A.; OBIOL, D.J.; FERMENTO, M.E.; FACCHINETTI, M.M.; LÓPEZ ROMERO, A.; ALONSO, E.N.

Bariloche

Simposio; III South American Symposium in Signal Transduction and Molecular Medicine (SISTAM); 2015

D Fraction is a proteoglycan extract obtained from the medicinal mushroom Grifola frondosa (Maitake). The antitumoral effect of D Fraction is mainly attributed to its immunomodulatory capacity and numerous studies have investigated the cellular and molecular mechanisms involved in this capacity. Recently, some reports have described a direct effect of D fraction on some neoplastic cells. We propose to demonstrate that D Fraction is also able to act directly on mammary tumor cells MDA-MB-231 and LM3, modulating their tumoral capacities and to elucidate the molecular mechanisms involved. We observed that D Fraction treatment decrease cell viability of MDA-MB-231 and LM3 in a dose and time-dependent manner, compared to vehicle-treated cells (WST-1 and subsequent manual counting; p