Antitumor evaluation of Neltuma caldenia aqueous extract in triple-negative breast cancer cells.

PEDERSOLI, A.P.; ALONSO, E.N.; IBARRA, A.; CLEMENTE, V.; GUTIERREZ, A.; MARINANGELI, P.; HAAG, L.M.; COLÓ, G.P.; FERMENTO, M.E.; CURINO, A.C.; FACCHINETTI, M.M.; FERRONATO, M.J.

Rosario

Congreso; 7ma Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2023); 2023

The medicinal potential of different species belonging to genus Neltuma have begun to be studied. Neltuma caldenia (Nc), commonly known as "Caldén", is a native tree from Argentina whose antitumor properties are still unexplored. Triple-Negative Breast Cancer (TNBC) is an aggressive subtype of BC that often presents high rates of relapses, metastases and with generally poor prognosis despite its therapeutic strategies. This work aims to investigate the antitumor activity of an Aqueous Extract (AE) obtained from Nc leaves in TNBC cells. To this end, TNBC cells (murine 4T1 and human MDA-MB-231) and a mammary non-malignant cell line (murine HC11) were used. Cells were treated with a range of concentrations (0.001-0.01- 0.1- 1.0- 2.5 mg/ml) of AENc or vehicle during 48 or 72 hours and cell viability was determined by colorimetric crystal violet and MTT assays. Then, in order to investigate the cellular mechanisms responsible of AENc action, cell cycle and apoptosis analyses were performed by flow cytometry with Propidium Iodide (PI) and, Annexin V / PI staining, respectively. Also, the effect of the AENc on cell migration was assessed by "wound healing" assays. The GraphPad Prism software was employed for all statistical analyses. The results show that AENc reduced 4T1 and MDA-MB-231 cell viability in a concentration-dependent manner (p0.05). The cell cycle analysis of 4T1 and MDA-MB-231 cells treated with AENcat 1 mg/ml for 48 hours displayed that it causes an arrest in S-phase compared to vehicletreated cells (p