PROTEIN KINASE D1 ACTIVITY IS ASSOCIATED TO MOUSE SPERM CAPACITATION

MARTÍNEZ LEÓN, EDUARDO ANTONIO; OSYCKA SALUT CLAUDIA ELENA; MARÍN-BRIGGILER, CLARA; JABLOÑSKI, MARTINA; GÓMEZ, MATÍAS; BUFFONE, MARIANO G.; REY, OSVALDO

Mar del Plata

Congreso; Reunión Conjunta de Biociencias 2023; 2023

TRABAJO SELECCIONADO PARA SAIC AWARD - Fundación BIGAND - Multidisciplinary call for young investigators.Sperm are specialized and transcriptionally inactive cells dependent on post-translational modifications, such as protein phosphorylation, to carry out their functions. These changes occur during the process called sperm capacitation. The protein kinase D family (PKDs) includes three serine/threonine kinases (PKD1, PKD2 and PKD3) being PKD1 the most studied and ubiquitous one. This family of kinases are involved in fundamental biological process including signal transduction, cytoskeleton remodeling, golgi transport and oxidative stress among other functions. Recent results from our laboratory indicate that PKD1 is present in the sperm of human, mice, equine and bovine. Since there are no previous reportsabout the presence and function(s) of any PKD in mammal sperm, we examined its distribution and function(s). We observed that: 1) PKD1 is present and catalytically active (p-PKD) in bovine, equine, mice, and human sperm (IIF/WB). 2) The capacitation process induces changes in kinase localization and activation levels, evaluated by super resolution microscopy in mouse sperm. 3) Mouse sperm incubated in the presence of specific PKD inhibitors (Kb142-70 and CRT0066101) before spermatozoa capacitation enhanced tyrosine-phosphorylation and phospho-PKA substrates proteins levels (p