Isolation and characterization of microsatellite loci in the rock scallop (Spondylus calcifer) (Bivalvia: Spondylidae) from the Northern Gulf of California, Mexico

ADRIAN MUNGUÍA-VEGA; GASPAR SORIA; TAD PFISTER; RICHARD CUDNEY-BUENO

CONSERVATION GENETICS

SPRINGER

Año: 2010 vol. 2 p. 51 - 54

1566-0621

Twelve microsatellite loci were isolated from an enriched genomic library of the rock scallop (Spondylus calcifer). One locus was monomorphic. Overall polymorphic loci, the mean numbers of alleles per locus at one locality was 9.6 (range 3–16), and the average observed and expected heterozygosities were 0.650 and 0.707, respectively. Three loci deviated from Hardy–Weinberg equilibrium, and from these, one locus had and excess of heterozygotes and the other two loci showed deficits of heterozygotes likely due to the presence of null alleles. No evidence of linkage disequilibrium was found among loci. These loci are the first microsatellites ever reported for the monotypic family Spondylidae, and will be useful to validate the predictions of oceanographic larval transport models and connectivity between patchy reefs within fishing areas and marine reserves in the northern Gulf of California, Mexico.