COMPARATIVE GENOMICS TO EXPLORE ZP EVOLUTION

CAMPOS, CLARA; PENNA, CLEMENTINA; BRUQUE, CARLOS DAVID; PISCIOTTANO, FRANCISCO; SARAGÜETA, PATRICIA

Congreso; Reunión Conjunta SAIC - SAI - AAFE - NANOMED-AR; 2021

SAI-SAIC-AAFE-Nanomed

COMPARATIVEGENOMICS TO EXPLORE ZP EVOLUTION MaríaClara Campos1,Clementina Penna1, David Bruque2,Francisco Pisciotanno1and Patricia Saragüeta11Instituto de Biología y Medicina Experimental - CABA, Provincia deBuenos Aires, Argentina 2Unidad de Conocimiento Traslacional Hospitalaria Patagónica,Hospital de Alta Complejidad SAMIC - El Calafate, Provincia de SantaCruz, ArgentinaThemammalian fertilization process has been extensively described byboth physiology and molecular biology. Zona Pellucida (ZP) andIZUMO1R oocyte proteins play a key role in this process, facilitatingthe interaction and fusion between the oocyte and the sperm,respectively. Their interactions with sperm counterparts mayconstitute a prezygotic reproductive isolation mechanism. The storyof protein evolution has greatly changed with the large number ofgenomesreportedas their sequencing advanced. We have improved the phylogeny ofgamete interaction proteins using chromosome-resolved genomeassembled by Hi-C long-range sequencing. ZP isoforms involved insperm-recognition, ZP3 and Zp2, structural cross-linker ZP proteins,ZP1 and Zp4, and IZUMO1R evolution analysis were studied amongmammals, carnivora, caniformia and feliformia groups. In order todetect adaptive changes in these proteins pairwise sequence identityand similarity analysis were performed using multiple sequencealignments. Positive selection signals were find using codemlsoftware from PAML package.Theconservation of all carnivora gamete interaction proteins wassignificantly higher (p-value < 0.05) in felids compared to canidsexcept for the case of ZP4. ZP3 and ZP2, display a similar pattern ofevolution along their phylogenies showing adaptive changes in thecanids subtree but not among felid species. On the other hand, bothZP1 and ZP4 display a different evolutionary history, showingsignatures of positive selection inside felids subtree. The fusionprotein IZUMO1R did not show positive selection among all studiedphylogenetic groups.Theseresults strengthen the idea that ZPs are proteins that provide a morespecific-specificity isolation than IZUMO1R, which is specialized infusion events.Altogether,our findings indicate that sperm-oocyte interaction and fusionproteins lack the degree of diversification necessary to fix aprezygotic reproductive isolation barrier in felidae.p { margin-bottom: 0.1in; direction: ltr; color: #000000; line-height: 115%; text-align: left; orphans: 2; widows: 2 }p.western { font-family: "Arial", serif; font-size: 11pt; so-language: es }p.cjk { font-family: "Arial"; font-size: 11pt; so-language: en-US }p.ctl { font-family: "Arial"; font-size: 11pt; so-language: ar-SA }