THE MICROENCAPSULATION OF THE FLAVONIOD GENISTEIN WITH SOLUBLE CHITOSAN ALLOWS EFFICIENT RELEASE IN COLON AND ATTENUATION OF OXIDATIVE STRESS DURING EXPERIMENTAL COLITIS

VANDEN BRABER NL; NOVOTNY NÚÑEZ I; BOHL LP; PORPORATTO C; MONTENEGRO MA; CORREA SG

Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2016

Genistein (G) is a flavonoid with antioxidant, anti-inflammatoryand anti-tumoral properties whose absorption occurs in the stomachand small intestine with significant loss of the bioactivity. Thehypothesis of the present work was that upon microencapsulation,G could modulate the inflammatory process in the classiccolitis model. Colitis was induced by administration of 3% (w /v) dextran sulfate sodium in drinking water for 5 days. G wasmicroencapsulated (MCG) in water soluble chitosan (WSCh) byspray-drying. Female C57BL/6 mice were divided into 5 groups:Control, Colitis, Colitis+G, Colitis+MCG and Colitis+WSCh. G (0.08mg per day), MCG (3.15 mg per day) and WSCh (3.07 mg perday) were administered orally for 7 days starting at the beginningof colitis induction and animals were clinically evaluated during10 days. The disease activity index (DAI) was calculated throughweight loss, consistency and bleeding stool. Colon was isolatedand processed for pro and anti-inflammatory cytokine analysisand superoxide dismutase (SOD), catalase and myeloperoxidase(MPO) activities. Statistical analyses were performed by ANOVA/Tukey. In MCG treated animals, the DAI was 70% lower than inColitis group (p