PLANKTONIC AND BIOFILM LIFESTYLES: SETUP AND PRELIMINARY RESULTS OF A METHODOLOGY TO STUDY THE INTERACTIONS OF MICROORGANISMS IN A BOVINE MASTITIS IN VITRO MODEL

BOHL LP; LERDA WE; CONESA A; ISAAC P; BRESER ML; VALLE J; TOLOSA DE TALAMONI NG; PORPORATTO C

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Inmunología (SAI); 2016

Staphylococcus aureus (SA) is the main pathogen isolated into intramammary infections from dairy cattle. SA has the ability to grow as biofilm, showing a reduction to antimicrobial susceptibility and to the recognition by the immune system. However, the response of epithelial cells to planktonic and biofilm cultures has not been characterized. The aim of this work was to study the immune response of bovine mammary epithelial cells to SA grown in planktonic and biofilms form. The study was performed using a cell line of bovine mammary epithelial (MAC-T) infected with different form of SA V329 grown. To determinate the interaction of biofilm grown, bacteria was cultures in a specific device consisting of a lid with 96 pegs. The CFU of planktonic and biofilm grown was normalized to co-cultures assays. MAC-T cells infected with planktonic and biofilms SA was evaluated to 2, 4, 6 and 24 h. Co-cultures interaction was evaluated by the internalization of SA (CFU) (2 h), pro-apoptotic (Bax) and anti-apoptotic (Bcl-2) mRNA expression (4 h) and IL-1β and IL-6 production in the supernatants by ELISA (2, 4, 6 and 24 h). Bacteria from planktonic cultures shown higher internalization than bacteria from biofilms cultures (p