MORPHOMETRICAL COMPARATIVE ANALYSIS BETWEEN ALCOHOLIC AND DIBETIC PAROTID SIALOSIS

BOHL L., MERLO C., GÓMEZ DE FERRARIS M. E. Y CARRANZA M.

Madrid, España

Congreso; XIII Congreso Nacional de la Sociedad Española de Histología e Ingeniería tisular. First international congress of Histology and Tissue Engineering; 2005

Sociedad Española de Histología e Ingeniería Tisular

Introduction: In previous studies, we observed that alcoholic and diabetic parotid sialosis show morphological characteristics that indicate the existence of a differential qualitative histopathological feature. Objective: With the purpose of comparing the morphometrical modifications among both pathologies, in parotid biopsies of patient with sialosis of diabetic and alcoholic etiology, we measured different variables in acini and ducts  and relative quantity of fatty tissue in stroma. Material and Methods: Samples of 5 biopsies of parotid of patient with diabetes mellitus type II and 12 of patient with alcoholic hepatic cirrhosis were used, both groups with clinical signs of glandular swelling. Seven samples of patient without these antecedents were used as control. Samples were embedded in paraffin and colored with routine techniques. Morphometrical study was carried out using a digital image analyzer (Optimas ã) attached to a video camera and an optic microscope (Axiophot Zeiss) with a magnification power of 400. Direct records were obtained in acini and ducts and with these data indirect parameters were calculated. In randomized microscopic field (40X) we calculated the relative quantity of fatty tissue. All data were processed using the “U” test of Mann Whitney (p <0.05). Results: From ten analyzed variables, only total ductal area and area occupied by the ductal epithelium showed significantly bigger values in the chronic alcoholic patients compared with diabetics. This last group, the stroma fatty infiltration was higher than in alcoholic individuals. Conclusions: Ductal system in alcoholics was more affected than in diabetics, due to the epithelial hypertrophy that contributed to increase the ductal size. The proportion of fatty tissue is another indicator that allows us to establish differences between both sialosis. These quantitative results indicate that the histopathological feature is not identical between alcoholic and diabetes sialosis. Partial Grant: Secyt, UNC Nº 123/04