PolyphosphatePoly(amine) Nanoparticles: Self-Assembly, Thermodynamics and Stability Studies

MARTINELLI, HERNAN; CUENCA, EZEQUIEL; RAMIREZ, MARIA DE LOS ANGELES; ANDREOZZI, PATRIZIA; HERNÁN RITACCO; MOYA, SERGIO ENRIQUE

Congreso; Nanomed-AR 2020; 2020

Asociación Argentina de Nanomedicinas

The interaction of the polyamine poly(allylamine hydrochloride) (PAH) with Na3PO4,Na4P2O7, Na5P3O10, Na6P6O18 and (NaPO3)26 salts, and the formation of polyamine phosphatenanoparticles (PANs) will be presented. DLS, ζ-potential measurements, AFM and TEM are usedto explore the formation, stability and pH sensitivity of PANs. An optimal concentration for PANformation is found for each phosphate salt in terms of most stable size and lowestpolydispersity index of the nanoparticles. Increasing the number of phosphate groups perphosphate salt results in a decrease in the concentration of phosphate ions needed for PANformation. PANs from Na6P6O18 and (NaPO3)26 salts show positive ζ-potentials for all phosphateconcentrations with values up to +80 mV. For smaller phosphate salts, ζ-potential changes frompositive to negative values as the concentration of phosphate increases. pH stability of PANswas found to depend on the type of phosphate used. PANs formed with small phosphatespresented a small window of stability with pH, from 8 to 9, while those formed with longphosphates were stable in more acidic pH environments, with (NaPO3)26 PANs stable still at pH =2. Our findings open multiple possibilities for fine tuning the pH sensitivity of PANs by varyingphosphate salts for potential applications in drug delivery.