Eugenol as anesthetic for Cnesterodon decemmaculatus (Cyprinodontiformes, Poeciliidae)

VERCELLINI, MARIA CLARA; REARTE, RAMIRO; LUCA DI CESARE; AYALA, MIGUEL ANGEL; MONTES MARTIN MIGUEL

LABORATORY ANIMALS

ROYAL SOC MEDICINE PRESS LTD

Lugar: LONDRES; Año: 2023

0023-6772

Anesthetics are commonly used in fish for surgery and to facilitate capture, handling and transport in aquaculture and experimental procedures. In research, the selection of the anesthetic depends on its propertiesand on the recovery time. Eugenol has been pointed out as an effective anesthetic for fish, alternative totraditional drugs. Although Cnesterodon decemmaculatus is widely used as a model in ecological bioassays,no anesthetic protocol has been reported for this species. The aim of the present study was to evaluate theinduction time (i.e. time to reach anesthetic stage VI) and recovery time in individuals of C. decemmaculatussubjected to eugenol at a fixed concentration, according to sex and pregnancy status. Forty-one fish weredivided into three groups: males, pregnant females and non-pregnant females. They were measured for totallength, standard length and weight, and the condition factor (K) was calculated. No significant differences ininduction and recovery times were found for sex, pregnancy status and K between groups. Results are acontribution toward the development protocol of a standard anesthetic protocol for C. decemmaculatus.