Chlamydia muridarum dampens the induction of protective Th1 immune responses by inducing IL10-producing B cells

SANCHEZ, LR; GODOY, GJ; GOROSITO SERRAN, M; MOTRICH, RD; GRUPPI, A; RIVERO, VE

Buenos Aires

Congreso; LXIII Reunión Anual de SAI, I Meeting LASID-SAI-FAIC, IV Meeting LASID, y II Meeting FAIC.; 2015

Sociedad Argentina de Inmunologia

Background: Chlamydia trachomatis is the most prevalent sexually transmitted bacterial infection worldwide. It has been postulated that Chlamydia spp. would evade host defenses through different strategies, e.g. the induction of anti-inflammatory cytokines like IL10. Herein, we evaluated the role of IL10-producing B cells in a model of male genital tract (MGT) infection with Chlamydia muridarum (Cm), a serovar that infects rodents and produces a similar disease to that observed in humans.Methods: Male NOD mice were inoculated with Cm in the meatus urethra and then euthanized at different times post infection. Bacterial burden in the MGT organs and cytokines produced by Cm-stimulated spleen or lymph node mononuclear cells (MNC) were analyzed.Results: qPCR analyses revealed higher bacterial burdens in prostate than in urethral tissues from infected NOD mice (p