Dioctophyme renale genome: new insights in nematode comparative genomics

MALDONADO, L.L.; GONZÁLEZ, P.; GIORELLO, N.; BLAXTER, M.; FRANCHINI, G.R.; KAMENETZKY, L.

Simposio; BIODIVERSITY GENOMICS 2021; 2021

Dioctophyme renale (Goeze,1782), Order Dorylaimidae, Family Dioctophymidae, known as ?giant kidney worm?,is the biggest parasitic nematode, adult females can reach up to 103 cm longaffecting several species of land mammals including humans. We generated high-coverage assembly combining short and longDNA reads and transcriptomes of dissected male and female parasites. Weassemble 234 Mb in 219 contigs with 46.8% GC and ~58% of repetitive regions. Benchmarking of universal single copy orthologsestimates a completeness of 92.9% usingeukaryota lineage dataset and 64.1% using nematoda lineage dataset. Similarvalues are found in related species such as Trichinellaspiralis and Trichuris murissuggesting the presence of Clade I specific protein repertoire. More than 20,000protein coding genes were functionally annotated. This genome information is highlyinformative for Clade I nematode which are still underrepresented in genomedatabases and will be placed in an evolutionary context by comparative genomicsanalysis.