Characterization of a local isolate of Mannheimia haemolytica

AGUSTIN FRANCHINI; GISELA R. FRANCHINI; JULIETA FERNANDEZ; FEDERICO SISTI

Mendoza

Congreso; Reunión anual Edición LVIII de la SAIB; 2022

Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular

Mannheimina haemolytica is a Gram-negative respiratory pathogen that is frequently isolated from Argentine feedlots. Symptoms are seen after a stressful situation such as transportation or diet changes. Infection by this bacterium causes weight loss and death in calves. In this work we present a strain of M. haemolytica isolated from the Buenos Aires province area. We performed a genome and phylogenetic analysis, and a search for the most preponderant virulence factors. In addition, we analyzed the formation of biofilm in glass tubes in static and shaking cultures. We confirmed LktA presence in the strain purifying the toxin from the supernatant in an overnight culture. Finally, a survival test was carried out, in which we used the nematode Caenorhabditis elegans as an infection model.