Outer membrane vesicles obtained from Bordetella pertussis Tohama expressing the lipid A deacylase PagL as a novel acellular vaccine candidate.

GAILLARD M. EMILIA; ASENSIO CRISTIAN; MORENO GRISELDA; BOTTERO DANIELA; ZURITA MARÍA EUGENIA; RUMBO MARTÍN; VAN DER LEY PETER; VAN DER ARK ARNO; HOZBOR DANIELA

VACCINE

ELSEVIER SCI LTD

Año: 2011 vol. 29 p. 1649 - 1656

0264-410X

In an effort to devise a safer and effective pertussis acelullar vaccine, outer membrane vesicles (OMVs) were engineered to decrease their endotoxicity. The pagL gene from Bordetella bronchiseptica, which encodes a lipid A 3-deacylase, was expressed in Bordetella pertussis strain Tohama I. The resulting OMVs, designated OMVs(BpPagL), contain tetra- instead of penta-acylated LOS, in addition to pertussis surface immunogens such as pertactin and pertussis toxin, as the wild type OMVs. The characterized pertussis OMVs(BpPagL) were used in murine B. pertussis intranasal (i.n.) challenge model to examine their protective capacity when delivered by i.n. routes. Immunized BALB/c mice were challenged with sublethal doses of B. pertussis. Significant differences between immunized animals and the PBS treated group were observed (p