Hot water-soluble polysaccharides from tetrasporic Pterocladia capillacea

MARÍA I. ERREA; MARÍA C. MATULEWICZ

PHYTOCHEMISTRY

Pergamon, Elsevier Science, Ltd.

Lugar: Oxford, Gran Bretaña; Año: 1996 vol. 42 p. 1071 - 1073

0031-9422

Tetrasporic Pterocladia capillacea was sequentially extracted with water at room temperature, 50°, 70° and 90°. The polysaccharides obtained by extraction at 90° were fractionated by ion-exchange chromatography on DEAE Sephadex A-50 (Cl−), eluting with water and solutions of increasing sodium chloride concentration; seven fractions were separated. The two major fractions, one eluted with water and the other with 0.1 M NaCl, comprised 66.9% of that recovered and were mainly agarose. Structural analysis of the fraction eluted with 0.7 M NaCl is consistent with a backbone formed mainly by alternating 3-linked â-D-galactose and 4-linked á-L-galactose residues, highly substituted with sulphate and having single stubs of xylose and galactose.