What makes A. guillouiae SFC 500-1a able to co- metabolize phenol and Cr(VI)? a proteomic approach.

ONTAÑÓN ORNELLA M; LANDI CLAUDIA; CARLEO ALFONSO; GAGLIARDI ASSUNTA; BIANCHI LAURA; GONZÁLEZ PAOLA S.; AGOSTINI ELIZABETH; BINI LUCA

JOURNAL OF HAZARDOUS MATERIALS.

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2018 vol. 354 p. 215 - 224

0304-3894

Acinetobacter guillouiae SFC 500-1A is an environmental bacterium able to efficiently co-remediate phenol and Cr(VI). To further understand the molecular mechanisms triggered in this strain during the bioremediation process,variations in the proteomic profile after treatment with phenol and phenol plus Cr(VI) were evaluated.The proteomic analysis revealed the induction of the β-ketoadipate pathway for phenol oxidation and theassimilation of degradation products through TCA cycle and glyoxylate shunt. Phenol exposure increased theabundance of proteins associated to energetic processes and ATP synthesis, but it also triggered cellular stress.The lipid bilayer was suggested as a target of phenol toxicity, and changing fatty acids composition seemed to bethe bacterial response to protect the membrane integrity. The involvement of two flavoproteins in Cr(VI) reductionto Cr(III) was also proposed. The results suggested the important role of chaperones, antioxidant responseand SOS-induced proteins in the ability of the strain to mitigate the damage generated by phenol and Cr(VI).This research contributes to elucidate the mechanisms involved in A. guillouiae SFC 500-1A tolerance and coremediationof phenol and Cr(VI). Such information may result useful not only to improve its bioremediationefficiency but also to identify putative markers of resistance in environmental bacteria.